![]() ![]() Furthermore, omentin-1 pretreatment reduced the excessive reactive oxygen species. ![]() Moreover, omentin-1 preconditioning for 1 h could protect MSCs against H 2O 2-induced apoptosis in a concentration-dependent manner. Results After treatment with omentin-1 (100–800 ng/ml), MSCs displayed a higher proliferative capacity with an increasing number of cells in the S and G2 phase of the cell cycle. The cytokine secretion was assessed by ELISA. ![]() Tube formation assay was used to test the angiogenesis role of conditioned medium from MSCs in vitro. Expression levels of Akt, FoxO3a, GSK-3β, and apoptosis- and cell cycle-associated proteins were detected by Western blotting. Hoechst 33342 dye was used to assess morphological changes of apoptosis. Cell cycle, apoptosis ratio, reactive oxygen species concentration, and mitochondrial membrane potential were detected by flow cytometry. Cell proliferation was assessed with CCK-8 and EdU assay. Methods MSCs were isolated from 60–80 g donor rats. 3.0 Limited Edition is now available! Needs registration. GP-Pro EX, has evolved for fast and effective screen development. Click the above file 'GPPROEX405100D1.exe' to download the file to the desired folder.
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